FcZero-rAb® Biotin Anti-Human FGFR4 Rabbit Recombinant Antibody

• Home
• Products
• Primary Antibodies
• Flow Cytometry Antibodies
• FcZero-rAb® Biotin Anti-Human FGFR4 Rabbit Recombinant Antibody (Biotin-FcA98383-2)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

FGFR4 FcZero-rAb^® Recombinant Antibody for FC

Cat No. Biotin-FcA98383-2

Clone No.242713H4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD334, EC:2.7.10.1, FGFR 4, FGFR-4, JTK2

• 

  FC experiment of HepG2 using Biotin-FcA98383-2

  1x10^6 HepG2 cells(left) or A431 cells (right) were surface stained with 0.25 ug Biotin Anti-Human FGFR4 Rabbit RecAb (Biotin-FcA98383-2, Clone:242713H4) (red), or 5 ul FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue) and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98383-2 targets FGFR4 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3510

  Product name: Recombinant Human FGFR4 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 22-369 aa of BC011847

  Sequence: LEASEEVELEPCLAPSLEQQEQELTVALGQPVRLCCGRAERGGHWYKEGSRLAPAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMIVLQNLTLITGDSLTSSNDDEDPKSHRDPSNRHSYPQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPTPTIRWLKDGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENAVGSIRYNYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTADINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPEEDPTWTAAAPEARYTD

• Full Name: fibroblast growth factor receptor 4
• Calculated Molecular Weight: 88 kDa
• GenBank Accession Number: BC011847
• Gene Symbol: FGFR4
• Gene ID (NCBI): 2264
• RRID: AB_3747052
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P22455
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Fibroblast growth factor receptor 4 (FGFR4), also known as CD334, is a member of a highly conserved tyrosine kinase family, along with FGFR1-3. This family consists of an intracellular tyrosine kinase domain, a single transmembrane domain, and extracellular ligand-binding domains (PMID:32492514). FGFR4 is the predominant FGFR isoform present in human hepatocytes. FGFR4 has been proposed to play a role in the observed induction of hepatocyte proliferation and carcinogenesis by FGF19; however, contradicting evidence proposing a protective role for FGFR4 in suppressing hepatoma progression has also been proposed(PMID:20018895). While the role of FGFR4 in cancer remains to be fully elucidated, several findings suggest that this receptor may be an important player in Hepatocellular carcinoma (HCC) development and/or progression (PMID:10336501).