FH Polyclonal antibody
FH Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC
Conjugate
Unconjugated
Cat no : 10966-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, A431 cells, HEK-293 cells, HepG2 cells, human liver tissue, L02 cells, mouse liver tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human prostate cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
IF | See 1 publications below |
Product Information
10966-1-AP targets FH in WB, IP, IHC, IF, FC applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FH fusion protein Ag1437 |
Full Name | fumarate hydratase |
Calculated molecular weight | 55 kDa |
Observed molecular weight | 45-50 kDa |
GenBank accession number | BC017444 |
Gene symbol | FH |
Gene ID (NCBI) | 2271 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts a tumor suppressor functions of FH in sporadic kidney cancer(PMID:21695080). This protein has 2 isoforms produced by alternative initiation.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for FH antibody 10966-1-AP | Download protocol |
IHC protocol for FH antibody 10966-1-AP | Download protocol |
IF protocol for FH antibody 10966-1-AP | Download protocol |
IP protocol for FH antibody 10966-1-AP | Download protocol |
FC protocol for FH antibody 10966-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Endocr Relat Cancer Cellular kinetics of MED12-mutant uterine leiomyoma growth and regression in vivo. |