FH Polyclonal antibody

FH Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10966-1-AP

Synonyms

FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1



Tested Applications

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, HepG2 cells, human liver tissue, L02 cells, mouse liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10966-1-AP targets FH in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FH fusion protein Ag1437
Full Name fumarate hydratase
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC017444
Gene Symbol FH
Gene ID (NCBI) 2271
RRIDAB_568948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts a tumor suppressor functions of FH in sporadic kidney cancer(PMID:21695080). This protein has 2 isoforms produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for FH antibody 10966-1-APDownload protocol
IHC protocol for FH antibody 10966-1-APDownload protocol
IF protocol for FH antibody 10966-1-APDownload protocol
IP protocol for FH antibody 10966-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Identification of SPOP related metabolic pathways in prostate cancer.

Authors - Min Yan
humanIF

Endocr Relat Cancer

Cellular kinetics of MED12-mutant uterine leiomyoma growth and regression in vivo.

Authors - Vanida Ann Serna
human,mouseWB,IHC

Pharmaceuticals (Basel)

Identification of a Difluorinated Alkoxy Sulfonyl Chloride as a Novel Antitumor Agent for Hepatocellular Carcinoma through Activating Fumarate Hydratase Activity

Authors - Jin Jin