FH Polyclonal antibody

FH Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10966-1-AP

Synonyms

FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1

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Validation Data Gallery

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  WB analysis of HeLa using 10966-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10966-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10966-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10966-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10966-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10966-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10966-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10966-1-AP

  IP result of anti-FH (IP:10966-1-AP, 4ug; Detection:10966-1-AP 1:1000) with HepG2 cells lysate 2400ug.

• 

  IHC staining of human prostate cancer using 10966-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10966-1-AP (FH antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 10966-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10966-1-AP (FH Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human liver using 10966-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10966-1-AP (FH Antibody) at dilution of 1:50(under 40x lens).

• 

  IF Staining of HepG2 using 10966-1-AP

  Immunofluorescent analysis of HepG2 cells, using FH antibody 10966-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 10966-1-AP

  Immunofluorescent analysis of HepG2 cells using 10966-1-AP (FH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10966-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug FH antibody (10966-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, HEK-293 cells, HepG2 cells, human liver tissue, L02 cells, mouse liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human prostate cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10966-1-AP targets FH in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FH fusion protein Ag1437
• Full Name: fumarate hydratase
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC017444
• Gene Symbol: FH
• Gene ID (NCBI): 2271
• RRID: AB_568948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts a tumor suppressor functions of FH in sporadic kidney cancer(PMID:21695080). This protein has 2 isoforms produced by alternative initiation.

Publications

Species	Application	Title
human	WB	Oncotarget; Identification of SPOP related metabolic pathways in prostate cancer.; Authors - Min Yan
human	IF	Endocr Relat Cancer; Cellular kinetics of MED12-mutant uterine leiomyoma growth and regression in vivo.; Authors - Vanida Ann Serna
human,mouse	WB,IHC	Pharmaceuticals (Basel); Identification of a Difluorinated Alkoxy Sulfonyl Chloride as a Novel Antitumor Agent for Hepatocellular Carcinoma through Activating Fumarate Hydratase Activity; Authors - Jin Jin