FHL2 Recombinant antibody

• 

  WB analysis using 86060-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86060-1-RR (FHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

FHL2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86060-1-RR

Clone No.250585E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

AAG11, DRAL, FHL 2, FHL-2, LIM domain protein DRAL

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Tested Applications

• Positive WB detected in: HeLa cells, PANC-1 cells, U2OS cells, hTERT-RPE1 cells, MG-63 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86060-1-RR targets FHL2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FHL2 fusion protein Ag29655
• Full Name: four and a half LIM domains 2
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC014397
• Gene Symbol: FHL2
• Gene ID (NCBI): 2274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14192
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FHL2(Four and a half LIM domains protein 2), also known as DRAL. It is located in cytoplasm and nucleus. The protein is expressed in skeletal muscle and heart. May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PMID: 28717008). The molecular weight of FHL2 is 32 kDa.