FJX1 Polyclonal antibody, PBS Only

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• FJX1 Polyclonal antibody, PBS Only (17417-1-PBS)

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  WB analysis of rat brain using 17417-1-AP (same clone as 17417-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17417-1-AP (FJX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17417-1-AP (same clone as 17417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17417-1-AP (same clone as 17417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 17417-1-AP (same clone as 17417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 17417-1-AP (same clone as 17417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17417-1-AP (same clone as 17417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17417-1-AP (same clone as 17417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

FJX1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 17417-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

17417-1-PBS targets FJX1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11385

  Product name: Recombinant human FJX1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 43-437 aa of BC049171

  Sequence: EDRLPRRPARSGGPAPAPRFPLPPPLAWDARGGSLKTFRALLTLAAGADGPPRQSRSEPRWHVSARQPRPEESAAVHGGVFWSRGLEEQVPPGFSEAQAAAWLEAARGARMVALERGGCGRSSNRLARFADGTRACVRYGINPEQIQGEALSYYLARLLGLQRHVPPLALARVEARGAQWAQVQEELRAAHWTEGSVVSLTRWLPNLTDVVVPAPWRSEDGRLRPLRDAGGELANLSQAELVDLVQWTDLILFDYLTANFDRLVSNLFSLQWDPRVMQRATSNLHRGPGGALVFLDNEAGLVHGYRVAGMWDKYNEPLLQSVCVFRERTARRVLELHRGQDAAARLLRLYRRHEPRFPELAALADPHAQLLQRRLDFLAKHILHCKAKYGRRSGT

• Full Name: four jointed box 1 (Drosophila)
• Calculated Molecular Weight: 437 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC049171
• Gene Symbol: FJX1
• Gene ID (NCBI): 24147
• RRID: AB_2878406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86VR8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Four-jointed box protein 1 (FJX1), also named as FLJ22416, is a 437 amino acid protein, which belongs to the FJX1 family. FJX1 is a secreted protein and acts as an inhibitor of dendrite and branching. FJX1 is important for growth and differentiation of legs and wings, and for proper development of the eyes. FAT4, an essential gene that has a key role in vertebrate PCP (planar cell polarity), represses FJX1 expression which may lead to cystic diseases in humans.