FKBP10/FKBP65 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 81567-1-RR (same clone as 81567-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81567-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81567-1-RR (same clone as 81567-1-PBS)

  WB result of FKBP10/FKBP65 antibody (81567-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP10/FKBP65 transfected HeLa cells. This data was developed using the same antibody clone with 81567-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 81567-1-RR (same clone as 81567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81567-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 81567-1-RR (same clone as 81567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81567-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81567-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81567-1-RR against Human FKBP10/FKBP65 were performed. The affinity constant is 32.4 pM.

• Featured Product
• KD/KO Validated

FKBP10/FKBP65 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 81567-1-PBS

Clone No.S94

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

FKBP10, FKBP65, FKBP 65, FKBP 10, FK506-binding protein 10

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Product Information

81567-1-PBS targets FKBP10/FKBP65 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2814

  Product name: Recombinant human FKBP10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 233-582 aa of BC016467

  Sequence: PFLAYGEKGYGTVIPPQASLVFHVLLIDVHNPKDAVQLETLELPPGCVRRAGAGDFMRYHYNGSLMDGTLFDSSYSRNHTYNTYIGQGYIIPGMDQGLQGACMGERRRITIPPHLAYGENGTGDKIPGSAVLIFNVHVIDFHNPADVVEIRTLSRPSETCNETTKLGDFVRYHYNCSLLDGTQLFTSHDYGAPQEATLGANKVIEGLDTGLQGMCVGERRQLIVPPHLAHGESGARGVPGSAVLLFEVELVSREDGLPTGYLFVWHKDPPANLFEDMDLNKDGEVPPEEFSTFIKAQVSEGKGRLMPGQDPEKTIGDMFQNQDRNQDGKITVDELKLKSDEDEERVHEEL

• Full Name: FK506 binding protein 10, 65 kDa
• Calculated Molecular Weight: 582 aa, 64 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC016467
• Gene Symbol: FKBP10
• Gene ID (NCBI): 60681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96AY3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.