FKBP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

FKBP3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11755-1-AP

Synonyms

25 kDa FK506 binding protein, 25 kDa FKBP, FK506 binding protein 3, FK506 binding protein 3, 25kDa, FKBP 25, FKBP 3, FKBP25, FKBP3, Immunophilin FKBP25, PPIase, PPIase FKBP3, Rotamase

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Validation Data Gallery

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  WB analysis of HeLa using 11755-1-AP

  WB result of FKBP3 antibody (11755-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP3 transfected HeLa cells.

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  WB analysis of PC-3 using 11755-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11755-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11755-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11755-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11755-1-AP (FKBP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells, A431 cells
• Positive IHC detected in: mouse brain tissue, human lung cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11755-1-AP targets FKBP3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FKBP3 fusion protein Ag2351
• Full Name: FK506 binding protein 3, 25kDa
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC020809
• Gene Symbol: FKBP3
• Gene ID (NCBI): 2287
• RRID: AB_2102983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cell Mol Med; FKBP3 aggravates the malignant phenotype of diffuse large B-cell lymphoma by PARK7-mediated activation of Wnt/β-catenin signalling; Authors - Xiaojing Xing ; KD Validated
human	IHC	BMC Cancer; Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma.; Authors - Yang Zhai