FKBP5 Monoclonal antibody, PBS Only

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  WB analysis using 67874-1-Ig (same clone as 67874-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 67874-1-Ig (same clone as 67874-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 67874-1-Ig (same clone as 67874-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67874-1-Ig (same clone as 67874-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67874-1-Ig (same clone as 67874-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

FKBP5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67874-1-PBS

Clone No.3B11F3

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

FKBP-51, 3B11F3, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, AIG6

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Product Information

67874-1-PBS targets FKBP5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FKBP5 fusion protein Ag5514
• Full Name: FK506 binding protein 5
• Calculated Molecular Weight: 457 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC042605
• Gene Symbol: FKBP5
• Gene ID (NCBI): 2289
• RRID: AB_2918632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13451
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FKBP5, also known as FKBP51, belongs to the family of immunophilins, FK506 binding proteins (FKBPs). FKBP5 is a negative regulator of GR activity and plays a key role in the termination of the stress response by GRs. FKBP5 limits GR function by decreasing ligand-binding sensitivity, thereby delaying nuclear translocation and reducing GR-dependent transcriptional activity. The known functions of FKBP5 include the regulation of steroid hormone receptors, inhibiting apoptosis in cancer cells, promoting Akt1 dephosphorylation, and inhibiting the Akt1 pathways, to name a few. FKBP5 is widely expressed and the calculated molecular weight is 51 kDa (PMID: 21119664, PMID: 34077736, PMID: 37154033).