FKBPL Monoclonal antibody

FKBPL Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2H10B10

Cat no : 66389-1-Ig

Synonyms

DIR1, FK506 binding protein like, FKBPL, NG7, WISP39

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Validation Data Gallery

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  WB analysis of HeLa using 66389-1-Ig

  Western blot analysis of FKBPL-specific in various cell lines using Proteintech antibody 66389-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66389-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66389-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66389-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66389-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66389-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66389-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 66389-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 66389-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66389-1-Ig (FKBPL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, Raji cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, ROS1728 cells, C6 cells, RAW 264.7 cells
• Positive IHC detected in: human breast cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66389-1-Ig targets FKBPL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FKBPL fusion protein Ag0112
• Full Name: FK506 binding protein like
• Calculated Molecular Weight: 349 aa, 38 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC004168
• Gene Symbol: FKBPL
• Gene ID (NCBI): 63943
• RRID: AB_2881765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 kDa and 48 kDa by western blot.

Publications

Species	Application	Title
human	WB	Cell Mol Life Sci; A placenta-on-a-chip model to determine the regulation of FKBPL and galectin-3 in preeclampsia; Authors - Sahar Masoumeh Ghorbanpour