FKBPL Monoclonal antibody

FKBPL Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2H10B10

Cat no : 66389-1-Ig

Synonyms

DIR1, FK506 binding protein like, FKBPL, NG7, WISP39



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, Raji cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, ROS1728 cells, C6 cells, RAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66389-1-Ig targets FKBPL in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FKBPL fusion protein Ag0112
Full Name FK506 binding protein like
Calculated molecular weight 349 aa, 38 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC004168
Gene symbol FKBPL
Gene ID (NCBI) 63943
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 kDa and 48 kDa by western blot.

Protocols

Product Specific Protocols
WB protocol for FKBPL antibody 66389-1-IgDownload protocol
IHC protocol for FKBPL antibody 66389-1-IgDownload protocol
IF protocol for FKBPL antibody 66389-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols