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FKBPL Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat no : MP03399-1

Synonyms

DIR1, FK506-binding protein-like, NG7, WAF-1/CIP1 stabilizing protein 39, WISp39

Validation Data Gallery

  • Sandwich ELISA standard curve of MP03399-1, Human FKBPL Recombinant Matched Antibody Pair - PBS only. 88015-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4783. 88015-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

    Sandwich ELISA standard curve of MP03399-1

    Sandwich ELISA standard curve of MP03399-1, Human FKBPL Recombinant Matched Antibody Pair - PBS only. 88015-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4783. 88015-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Plasma of six individual healthy human donors was measured. The FKBPL concentration of detected samples was determined to be 0.6 ng/mL with a range of 0.2-1.1 ng/mL.

    Sandwich ELISA sample test of MP03399-1

    Plasma of six individual healthy human donors was measured. The FKBPL concentration of detected samples was determined to be 0.6 ng/mL with a range of 0.2-1.1 ng/mL.

  • The mean FKBPL concentration was determined to be 2.5 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 1.4 ng/mL in HT-29 cell extract based on a 1.5 mg/mL extract load, 3.4 ng/mL in A2780 cell extract based on a 1.2 mg/mL extract load and 2.5 ng/mL in OVCAR-3 cell extract based on a 2.5 mg/mL extract load.

    Sandwich ELISA sample test of MP03399-1

    The mean FKBPL concentration was determined to be 2.5 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 1.4 ng/mL in HT-29 cell extract based on a 1.5 mg/mL extract load, 3.4 ng/mL in A2780 cell extract based on a 1.2 mg/mL extract load and 2.5 ng/mL in OVCAR-3 cell extract based on a 2.5 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP03399-1 targets FKBPL in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: FKBPL Recombinant monoclonal antibody, PBS Only (Capture) 88015-4-PBS (260148B12). 100 μg. Concentration 1 mg/ml.

Detection antibody: FKBPL Recombinant monoclonal antibody, PBS Only (Detector) 88015-3-PBS (260148B10). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Eg4783

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 88015-4-PBS
Tested Reactivity human
Immunogen FKBPL fusion protein Eg4783
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name FK506 binding protein like
Synonyms DIR1, FK506-binding protein-like, NG7, WAF-1/CIP1 stabilizing protein 39, WISp39
GenBank accession numberBC011966
Gene symbol FKBPL
UniprotQ9UIM3
Gene ID (NCBI) 63943
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 88015-3-PBS
Tested Reactivity human
Immunogen FKBPL fusion protein Eg4783
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name FK506 binding protein like
Synonyms DIR1, FK506-binding protein-like, NG7, WAF-1/CIP1 stabilizing protein 39, WISp39
GenBank accession numberBC011966
Gene symbol FKBPL
UniprotQ9UIM3
Gene ID (NCBI) 63943
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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