ARHGEF40 Polyclonal antibody

ARHGEF40 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20110-1-AP

Synonyms

ARHGEF40, FLJ10357, hypothetical protein FLJ10357, Protein SOLO, SOLO

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 20110-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20110-1-AP (ARHGEF40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20110-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20110-1-AP (ARHGEF40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 20110-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20110-1-AP (ARHGEF40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20110-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20110-1-AP (ARHGEF40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20110-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20110-1-AP (ARHGEF40 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20110-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20110-1-AP (ARHGEF40 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 20110-1-AP

  Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 20110-1-AP (ARHGEF40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells, mouse brain tissue, Neuro-2a cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20110-1-AP targets ARHGEF40 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARHGEF40 fusion protein Ag13886
• Full Name: hypothetical protein FLJ10357
• Calculated Molecular Weight: 414 aa, 46 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC000084
• Gene Symbol: FLJ10357
• Gene ID (NCBI): 55701
• RRID: AB_10695049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.