ChromoTek FLT3 VHH, recombinant binding protein for 2x Cys conjugation

Validation Data Gallery

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  The affinity of anti-human FLT3 recombinant VHH towards human FLT3 was determined using biolayer interferometry (BLI). Biotinylated, recombinant human FLT3 was immobilized on Streptavidin biosensors and assayed with 1.5 to 100 nM of FITC Plus conjugated-FLT3 VHH (FITC-flt). Fit indicates a 1:1 binding model fitted to the data.

Alpaca anti-FLT3 VHH, purified recombinant binding protein. Suitable for for cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteins reduced.

Cat No. fltCys2

Host/Type

Alpaca VHH

Reactivity

Human

Applications

Conjugation

Conjugate

Unconjugated

Synonyms

FLT3-Nanobody, FLT3-Single domain antibody (sdAb), Receptor-type tyrosine-protein kinase, FL cytokine receptor, CD135, FLK2, FLK-2 STK1, STK-1, FLT3, FLT-3

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Product Information

Alpaca anti-FLT3 VHH, purified recombinant binding protein. Suitable for for cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteins reduced.

• Applications: Conjugation
• Reactivity: Human
• Conjugate: Unconjugated
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity: Picomolar range, below the assay limit (biolayer interferometry)
• Molecular Weight: 15.433 kDa
• Form: Liquid
• RRID: AB_3101944
• Storage Buffer: 10 mM HEPES, 500 mM NaCl, pH 7.0, 1 mM TCEP, 0.09% sodium azide
• Storage Condition: Store at -20°C
• Background: FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

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