FMO4 Monoclonal antibody

FMO4 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2D12E10

Cat no : 67881-1-Ig

Synonyms

Dimethylaniline oxidase 4, FMO 4, FMO2, FMO4

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Validation Data Gallery

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  WB analysis using 67881-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HuH-7 using 67881-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse liver using 67881-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse liver using 67881-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HuH-7 using 67881-1-Ig

  1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: rabbit liver tissue, HuH-7 cells, pig liver tissue, rat liver tissue, mouse liver tissue
• Positive IF detected in: mouse liver tissue
• Positive FC detected in: HuH-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67881-1-Ig targets FMO4 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FMO4 fusion protein Ag7935
• Full Name: flavin containing monooxygenase 4
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC002780
• Gene Symbol: FMO4
• Gene ID (NCBI): 2329
• RRID: AB_2918638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.