FMO4 Monoclonal antibody

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  WB analysis using 67881-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HuH-7 using 67881-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse liver using 67881-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse liver using 67881-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HuH-7 using 67881-1-Ig

  1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

FMO4 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Cat No. 67881-1-Ig

Clone No.2D12E10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF-P, FC (Intra), ELISA

Synonyms

2D12E10, Dimethylaniline monooxygenase [N-oxide-forming] 4, EC:1.14.13.8, FMO2, Hepatic flavin-containing monooxygenase 4

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Tested Applications

• Positive WB detected in: rabbit liver tissue, HuH-7 cells, pig liver tissue, rat liver tissue, mouse liver tissue
• Positive IF-P detected in: mouse liver tissue
• Positive FC (Intra) detected in: HuH-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67881-1-Ig targets FMO4 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7935

  Product name: Recombinant human FMO4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 24-354 aa of BC002780

  Sequence: EDLEPTCFERSDDIGGLWKFTESSKDGMTRVYKSLVTNVCKEMSCYSDFPFHEDYPNFMNHEKFWDYLQEFAEHFDLLKYIQFKTTVCSITKRPDFSETGQWDVVTETEGKQNRAVFDAVMVCTGHFLNPHLPLEAFPGIHKFKGQILHSQEYKIPEGFQGKRVLVIGLGNTGGDIAVELSRTAAQVLLSTRTGTWVLGRSSDWGYPYNMMVTRRCCSFIAQVLPSRFLNWIQERKLNKRFNHEDYGLSITKGKKAKFIVNDELPNCILCGAITMKTSVIEFTETSAVFEDGTVEENIDVVIFTTGYTFSFPFFEEPLKSLCTKKIFLYKQ

• Full Name: flavin containing monooxygenase 4
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC002780
• Gene Symbol: FMO4
• Gene ID (NCBI): 2329
• RRID: AB_2918638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P31512
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.