FNDC3B Recombinant antibody, PBS Only (Detector)

• 

  WB analysis using 86272-1-RR (same clone as 86272-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86272-1-RR (FNDC3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86272-1-PBS in a different storage buffer formulation.

• 

  Sample test of MP02305-1

  The mean FNDC3B concentration was determined to be 70.0 ng/mL in HepG2 cell extract based on a 0.9 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP02305-1

  Cytometric bead array standard curve of MP02305-1, FNDC3B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86272-2-PBS. Detection antibody: 86272-1-PBS. Standard: Ag18357. Range: 3.125-200 ng/mL.

FNDC3B Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 86272-1-PBS

Clone No.250827H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

FAD104, fibronectin type III domain containing 3B, Fibronectin type III domain-containing protein 3B, HCV NS5A-binding protein 37, UNQ2421/PRO4979/PRO34274

---

Product Information

86272-1-PBS targets FNDC3B as part of a matched antibody pair:

MP02305-1: 86272-2-PBS capture and 86272-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FNDC3B fusion protein Ag18357
• Full Name: fibronectin type III domain containing 3B
• Calculated Molecular Weight: 1204 aa, 133 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC039297
• Gene Symbol: FNDC3B
• Gene ID (NCBI): 64778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q53EP0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fibronectin type III domain-containing protein 3B(FNDC3B), also named FAD104, belongs to the FNDC3 family. NDC3B is a positive regulator of adipocyte differentiation and is expressed at an early stage of adipocyte differentiation. FNDC3B is an endoplasmic reticulum transmembrane protein with a single transmembrane domain at the C terminus preceded by nine repeated fibronectin type III domains(PMID: 32966780). Due to the ability of the fibronectin type III domain to bind to a variety of proteins, FNDC3B plays a crucial role in cell adhesion, proliferation, and growth signaling. FNDC3B is abnormally expressed in a variety of human cancers, including hepatocellular carcinoma, acute myeloid leukemia, colorectal carcinoma, and cervical carcinoma(PMID: 36275754).