Tested Applications
| Positive WB detected in | HeLa cells, mouse kidney tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29472-1-AP targets FOLR1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | folate receptor 1 (adult) |
| Calculated Molecular Weight | 257 aa, 30 kDa |
| Observed Molecular Weight | 36-38 kDa |
| GenBank Accession Number | BC002947 |
| Gene Symbol | FOLR1 |
| Gene ID (NCBI) | 2348 |
| RRID | AB_2918313 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15328 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family (PMID:15094207; 23528302). The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma (PMID: 23528302; 23357463).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FOLR1 antibody 29472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FOLR1 Polyclonal antibody (catalog number 29472-1-AP) has 1 total citation. It appears in the journal International Journal of Molecular Sciences (2024), in a study on cerebrospinal fluid accumulation, ventricular enlargement, and cerebral folate metabolic errors in neuropsychiatric conditions. The associated research field is neuroscience/neuropsychiatry.
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Accumulation of Cerebrospinal Fluid, Ventricular Enlargement, and Cerebral Folate Metabolic Errors Unify a Diverse Group of Neuropsychiatric Conditions Affecting Adult Neocortical Functions. |
Reviews
What customers say
The single review for 29472-1-AP is a 5-star rating. The user reported excellent performance in Western Blot, describing it as a "great antibody" with a "very strong signal." It was tested on Hela cells as well as mouse liver, kidney, and brain tissues, using a 1:8000 dilution. Overall, the feedback highlights strong, reliable signal and broad tissue compatibility.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (02-14-2023) | Great antibody. Very strong signal.
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