FOXM1 Polyclonal antibody, PBS Only

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• FOXM1 Polyclonal antibody, PBS Only (13147-1-PBS)

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  WB analysis of HeLa using 13147-1-AP (same clone as 13147-1-PBS)

  WB result of FOXM1 antibody (13147-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXM1 transfected HeLa cells. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

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  WB analysis using 13147-1-AP (same clone as 13147-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13147-1-AP (same clone as 13147-1-PBS)

  Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13147-1-AP (same clone as 13147-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13147-1-AP (same clone as 13147-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 13147-1-AP (same clone as 13147-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 13147-1-AP (same clone as 13147-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 13147-1-AP (same clone as 13147-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 13147-1-AP (same clone as 13147-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 13147-1-AP (same clone as 13147-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 13147-1-AP (same clone as 13147-1-PBS)

  IP result of anti-FOXM1 (IP:13147-1-AP, 4ug; Detection:13147-1-AP 1:1000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

FOXM1 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 13147-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

M-phase phosphoprotein 2, HNF-3/fork-head homolog 11, HFH-11, HFH11, Forkhead-related protein FKHL16

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Product Information

13147-1-PBS targets FOXM1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3729

  Product name: Recombinant human FOXM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 447-748 aa of BC006192

  Sequence: EEIQPGEEMPHLARPIKVESPPLEEWPSPAPSFKEESSHSWEDSSQSPTPRPKKSYSGLRSPTRCVSEMLVIQHRERRERSRSRRKQHLLPPCVDEPELLFSEGPSTSRWAAELPFPADSSDPASQLSYSQEVGGPFKTPIKETLPISSTPSKSVLPRTPESWRLTPPAKVGGLDFSPVQTPQGASDPLPDPLGLMDLSTTPLQSAPPLESPQRLLSSEPLDLISVPFGNSSPSDIDVPKPGSPEPQVSGLAANRSLTEGLVLDTMNDSLSKILLDISFPGLDEDPLGPDNINWSQFIPELQ

• Full Name: forkhead box M 1
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 84-110 kDa
• GenBank Accession Number: BC006192
• Gene Symbol: FOXM1
• Gene ID (NCBI): 2305
• RRID: AB_2106213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q08050
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).