FOXN1 Monoclonal antibody

FOXN1 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1D8B12

Cat no : 66337-1-Ig

Synonyms

FKHL20, forkhead box N1, Forkhead box protein N1, FOXN1, RONU, WHN

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Validation Data Gallery

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  WB analysis of Raji using 66337-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66337-1-Ig (FOXN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66337-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66337-1-Ig (FOXN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of A549 using 66337-1-Ig

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human FOXN1 (66337-1-Ig, Clone:1D8B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells
• Positive FC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66337-1-Ig targets FOXN1 in WB, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FOXN1 fusion protein Ag18207
• Full Name: forkhead box N1
• Calculated Molecular Weight: 648 aa, 69 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC146539
• Gene Symbol: FOXN1
• Gene ID (NCBI): 8456
• RRID: AB_2881717
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXN1, also named as Forkhead box protein N1, is a 648 amino acid protein, which contains 1 fork-head DNA-binding domain and localizes in the nucleus. FOXN1 as transcriptional regulator regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. FOXN1 acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality.