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CoraLite® Plus 488-conjugated FOXN1 Monoclonal antibody

FOXN1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D8B12

Cat no : CL488-66337

Synonyms

FKHL20, forkhead box N1, Forkhead box protein N1, FOXN1, RONU, WHN

Validation Data Gallery

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  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated FOXN1 Monoclonal ant (CL488-66337)

    FC experiment of A549 using CL488-66337

    1X10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human FOXN1 (CL488-66337, Clone:1D8B12) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66337 targets FOXN1 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FOXN1 fusion protein Ag18207
Full Name forkhead box N1
Calculated Molecular Weight 648 aa, 69 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC146539
Gene Symbol FOXN1
Gene ID (NCBI) 8456
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXN1, also named as Forkhead box protein N1, is a 648 amino acid protein, which contains 1 fork-head DNA-binding domain and localizes in the nucleus. FOXN1 as transcriptional regulator regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. FOXN1 acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality.