FRS2 Polyclonal antibody, PBS Only

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• FRS2 Polyclonal antibody, PBS Only (11503-1-PBS)

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  WB analysis using 11503-1-AP (same clone as 11503-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11503-1-AP (FRS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11503-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 11503-1-AP (same clone as 11503-1-PBS)

  WB result of FRS2 antibody (11503-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FRS2 transfected SH-SY5Y cells. This data was developed using the same antibody clone with 11503-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 11503-1-AP (same clone as 11503-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11503-1-AP (FRS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11503-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11503-1-AP (same clone as 11503-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11503-1-AP (FRS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11503-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11503-1-AP (same clone as 11503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11503-1-AP (FRS2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11503-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11503-1-AP (same clone as 11503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11503-1-AP (FRS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11503-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11503-1-AP (same clone as 11503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11503-1-AP (FRS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11503-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 11503-1-AP (same clone as 11503-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using FRS2 antibody (11503-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11503-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

FRS2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 11503-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FGFR substrate 2, FGFR-signaling adaptor SNT, FRS2A, FRS2alpha, SNT

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Product Information

11503-1-PBS targets FRS2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2052

  Product name: Recombinant human FRS2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC021562

  Sequence: MGSCCSCPDKDTVPDNHRNKFKVINVDDDGNELGSGIMELTDTELILYTRKRDSVKWHYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEELFNMLQEIMQNNSINVVEEPVVERNNHQTELEVPRTPRTPTTPGFAAQNLPNGYPRYPSFGDASSHPSSRHPSVGSARLPSVGEESTHPLLVAEEQVHTYVNTTGVQEERKNRTSVHVPLEARVSNAESSTPKEEPSSIEDRDPQILLEPEGVKFVLGPTPVQKQLMEKEKLEQLGRDQVSGSGANNTEWDTGYDSDERRDAPS

• Full Name: fibroblast growth factor receptor substrate 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC021562
• Gene Symbol: FRS2
• Gene ID (NCBI): 10818
• RRID: AB_2262942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WU20
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.