FSD2 Polyclonal antibody

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FSD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25609-1-AP Copied!

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

FSD2, RP11 127F21, SPRYD1

• 

  WB analysis of mouse heart using 25609-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 25609-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat heart using 25609-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 25609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25609-1-AP (FSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 25609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25609-1-AP (FSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25609-1-AP targets FSD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22229

  Product name: Recombinant human FSD2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC130569

  Sequence: MEEESGEELGLDRSTPKDFHFYHMDLYDSEDRLHLFPEENTRMRKVVQAEMANESRGAGDGKAQRDLQEEVDELVHLYGLEDDHELGDEFVDENIPRTGVSEYPPYMMKRRDPAREQRDWRLSGEAAEAEDLGFGGWGSAGQCQDLREAYRYTHGRASEEYECYVIPEEEDEEEAADVFCVTCKTPIRAFQKVFDEHKEHEVIPLNEALESAKDEIHKNMYKLEKQIIEMENFANHLEEVFITVEENFGKQEQNFESHYNEILETLAQKYEEKIQALGEKKKEKLEALYGQLVSCGENLDTCKELMETIEEMCHEEKVDFIKDAVAMADRLGKFLKTKTDVEISAQPEFE

• Full Name: fibronectin type III and SPRY domain containing 2
• Calculated Molecular Weight: 749 aa, 85 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC130569
• Gene Symbol: FSD2
• Gene ID (NCBI): 123722
• RRID: AB_2880159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A1L4K1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The product received a single 5-star review from a researcher who used it for Western Blot on mouse heart tissue at a 1:1000 dilution. The reviewer reported that it worked very well, indicating reliable performance and clear results in their application. Overall, the feedback is positive, with the user expressing satisfaction with the antibody's effectiveness for Western Blot experiments.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Zee (Verified Customer) (01-28-2020)

It worked very well with western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue