FSTL1 Polyclonal antibody

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• FSTL1 Polyclonal antibody (20182-1-AP)

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• KD/KO Validated

FSTL1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 20182-1-AP Copied!

Publications (31)

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Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

follistatin like 1, Follistatin like protein 1, Follistatin related protein 1, Follistatin-like protein 1, Follistatin-related protein 1

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  WB analysis of HeLa using 20182-1-AP

  WB result of FSTL1 antibody (20182-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FSTL1 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis using 20182-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20182-1-AP (FSTL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 20182-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20182-1-AP (FSTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 20182-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 20182-1-AP (FSTL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 20182-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20182-1-AP (FSTL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 20182-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 20182-1-AP (FSTL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 20182-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20182-1-AP (FSTL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of A2780 using 20182-1-AP

  IP result of anti-FSTL1 (IP:20182-1-AP, 4ug; Detection:20182-1-AP 1:2000) with A2780 cells lysate 960 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20182-1-AP (FSTL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20182-1-AP (FSTL1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20182-1-AP (FSTL1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20182-1-AP (FSTL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20182-1-AP (FSTL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20182-1-AP (FSTL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat colon using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat colon using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A2780 cells, HeLa cells, human placenta tissue, A431 cells, C6 cells
• Positive IP detected in: A2780 cells
• Positive IHC detected in: human colon tissue, human placenta tissue, rat colon tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20182-1-AP targets FSTL1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14057

  Product name: Recombinant human FSTL1 protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1-308 aa of BC000055

  Sequence: MWKRWLALALALVAVAWVRAEEELRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSVSPSASPVVCYQSNRDELRRRIIQWLEAEIIPDGWFSKGSNYSEILDKYFKNFDNGDSRLDSSEFLKFVEQNETAINITTYPDQENNKLLRGLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCACGNWVCTAMTCDGKNQKGAQTQTEEEMTRYVQELQKHQETAEKTKRVSTKEI

• Full Name: follistatin-like 1
• Calculated Molecular Weight: 308 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC000055
• Gene Symbol: FSTL1
• Gene ID (NCBI): 11167
• RRID: AB_10666439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12841
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Follistatin-like 1 (FSTL1), initially discovered as a TGF-β1-induced gene, encodes a 308 amino acid secreted 45-55 kDa glycoprotein with a follistatin domain and two non-functional calcium-binding motifs. FSTL1 has been reported to be involved in the fate determination and maturation of epithelial cells. Ablation of the FSTL1 gene in the mouse results in several structural developmental defects and neonatal lethality due to respiratory failure, demonstrating its functional importance. FSTL1 has been reported to exhibit both pro- and anti-inflammatory actions, with a specific anti-apoptotic and protective effect in cardiac or renal cell injury. Zwijsen et al. (1994) detected several isoforms of FSTL1 with molecular masses of 40 to 48 kD which differs from the 50- to 55-kD products detected by Tanaka et al. (1998) (OMIM). (PMID: 26386648; PMID: 23723173; PMID: 20176958).

Publications

What published studies show

20182-1-AP is an anti-FSTL1 antibody with 31 total citations spanning journals such as Nature Communications, Advanced Science, Science Advances, Cancer Research, Journal of Experimental Medicine, EMBO Molecular Medicine, and Cell Death & Disease. Associated research fields include oncology (lung, colorectal, gastric, bladder, and breast cancer), osteoarthritis, pulmonary fibrosis, cardiovascular disease, inflammation, neuroprotection, viral infection, and muscular dystrophy.

Automatically generated from the 31 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Signal Transduct Target Ther; Human adipose and umbilical cord mesenchymal stem cell-derived extracellular vesicles mitigate photoaging via TIMP1/Notch1; Authors - Huan Zhang
human	WB	Cancer Res; Follistatin-like Protein 1 Inhibits Lung Cancer Metastasis by Preventing Proteolytic Activation of Osteopontin.; Authors - Jean Chiou ; KD Validated
mouse	IHC	Bone Res; Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx-/- mice.; Authors - Junxin Lin
rat,human	WB,IHC	Nat Commun; Denosumab attenuates knee osteoarthritis progression by inhibiting synovial inflammation via the RANK/TRAF6/FSTL1 signalling.; Authors - Yuxiang Hu
mouse	WB,IF	Adv Sci (Weinh); Activating the Osteoblastic USP26 Pathway Alleviates Multi-Organ Fibrosis by Decreasing Insulin Resistance.; Authors - Jiyuan Tang
human, mouse	IHC,IF	Adv Sci (Weinh); FSTL1 Orchestrates Metabolic-Epigenetic Crosstalk: Glycolysis-Dependent H3K18 Lactylation Drives Cartilage Fibrosis in Osteoarthritis.; Authors - Feng Lu