FSTL1 Monoclonal antibody

FSTL1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G5G1

Cat no : 68528-1-Ig

Synonyms

FLJ50214, FLJ52277, follistatin like 1, Follistatin like protein 1, Follistatin related protein 1, FRP, FSL1, FSTL1

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Validation Data Gallery

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  WB analysis using 68528-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68528-1-Ig (FSTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cellls, A2780 cells, HeLa cells, U2OS cells, JAR cells, C6 cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68528-1-Ig targets FSTL1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: FSTL1 fusion protein Ag33795
• Full Name: follistatin-like 1
• Calculated Molecular Weight: 308 aa, 35 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC000055
• Gene Symbol: FSTL1
• Gene ID (NCBI): 11167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Follistatin-like 1 (FSTL1), initially discovered as a TGF-β1-induced gene, encodes a 308 amino acid secreted 45-55 kDa glycoprotein with a follistatin domain and two non-functional calcium-binding motifs. FSTL1 has been reported to be involved in the fate determination and maturation of epithelial cells. Ablation of the FSTL1 gene in the mouse results in several structural developmental defects and neonatal lethality due to respiratory failure, demonstrating its functional importance. FSTL1 has been reported to exhibit both pro- and anti-inflammatory actions, with a specific anti-apoptotic and protective effect in cardiac or renal cell injury. Zwijsen et al. (1994) detected several isoforms of FSTL1 with molecular masses of 40 to 48 kD which differs from the 50- to 55-kD products detected by Tanaka et al. (1998) (OMIM).