FSTL1 Recombinant monoclonal antibody

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  WB analysis using 86444-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86444-1-RR (Fstl1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

FSTL1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86444-1-RR

Clone No.251240E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Follistatin-like protein 1, Follistatin-related protein 1, Frp, Fstl, TGF-beta-inducible protein TSC-36

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Tested Applications

• Positive WB detected in: 4T1 cells, C6 cells, mouse embryo tissue, A2780 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86444-1-RR targets FSTL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2859

  Product name: Recombinant Mouse FSTL1 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 19-306 aa of NM_008047.5

  Sequence: EEEPRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSASPSASPVVCYQANRDELRRRLIQWLEAEIIPDGWFSKGSNYSEILDKYFKSFDNGDSHLDSSEFLKFVEQNETAINITTYADQENNKLLRSLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCSCGHWVCTAMTCDGKNQKGVQTHTEEEKTGYVQELQKHQGTAEKTKKVNTKEI

• Full Name: follistatin-like 1
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35-45 kDa
• GenBank Accession Number: NM_008047.5
• Gene Symbol: Fstl1
• Gene ID (NCBI): 14314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q62356
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Follistatin-like 1 (FSTL1), initially discovered as a TGF-β1-induced gene, encodes a 308 amino acid secreted 45-55 kDa glycoprotein with a follistatin domain and two non-functional calcium-binding motifs. FSTL1 has been reported to be involved in the fate determination and maturation of epithelial cells. Ablation of the FSTL1 gene in the mouse results in several structural developmental defects and neonatal lethality due to respiratory failure, demonstrating its functional importance. FSTL1 has been reported to exhibit both pro- and anti-inflammatory actions, with a specific anti-apoptotic and protective effect in cardiac or renal cell injury. Zwijsen et al. (1994) detected several isoforms of FSTL1 with molecular masses of 40 to 48 kD, which differ from the 50- to 55-kD products detected by Tanaka et al. (1998) (OMIM). (PMID: 26386648; PMID: 23723173; PMID: 20176958).