Mouse FSTL1 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 86444-1-RR (same clone as 86444-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86444-1-RR (FSTL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86444-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02461-2

  The mean FSTL1 concentration was determined to be 9.20 ng/mL in mouse brain tissue extract based on a 6.6 mg/mL extract load.

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  Sandwich ELISA sample test of MP02461-2

  Serum of six mice was measured. The mouse FSTL1 concentration of detected samples was determined to be 64.09 ng/mL with a range of 43.91 - 83.14 ng/mL.

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  Sandwich ELISA standard curve of MP02461-2

  Sandwich ELISA standard curve of MP02461-2, Mouse FSTL1 Recombinant Matched Antibody Pair - PBS only. 86444-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2859. 86444-3-PBS was HRP conjugated as the detection antibody. Range: 97.7-6250 pg/mL

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  Cytometric bead array sample test of MP02461-1

  The mean FSTL1 concentration was determined to be 19.3 ng/mL in Mouse brain tissue extract based on a 7.0 mg/mL extract load.

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  Cytometric bead array standard curve of MP02461-1

  Cytometric bead array standard curve of MP02461-1, MOUSE FSTL1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86444-1-PBS. Detection antibody: 86444-2-PBS. Standard: Eg2859. Range: 1.562-200 ng/mL.

FSTL1 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 86444-1-PBS

Clone No.251240E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

Follistatin-like protein 1, Follistatin-related protein 1, Frp, Fstl, TGF-beta-inducible protein TSC-36

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Product Information

86444-1-PBS targets FSTL1 as part of a matched antibody pair:

MP02461-1: 86444-1-PBS capture and 86444-2-PBS detection (validated in Cytometric bead array)

MP02461-2: 86444-1-PBS capture and 86444-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2859

  Product name: Recombinant Mouse FSTL1 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 19-306 aa of NM_008047.5

  Sequence: EEEPRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSASPSASPVVCYQANRDELRRRLIQWLEAEIIPDGWFSKGSNYSEILDKYFKSFDNGDSHLDSSEFLKFVEQNETAINITTYADQENNKLLRSLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCSCGHWVCTAMTCDGKNQKGVQTHTEEEKTGYVQELQKHQGTAEKTKKVNTKEI

• Full Name: follistatin-like 1
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35-45 kDa
• GenBank Accession Number: NM_008047.5
• Gene Symbol: Fstl1
• Gene ID (NCBI): 14314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q62356
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Follistatin-like 1 (FSTL1), initially discovered as a TGF-β1-induced gene, encodes a 308 amino acid secreted 45-55 kDa glycoprotein with a follistatin domain and two non-functional calcium-binding motifs. FSTL1 has been reported to be involved in the fate determination and maturation of epithelial cells. Ablation of the FSTL1 gene in the mouse results in several structural developmental defects and neonatal lethality due to respiratory failure, demonstrating its functional importance. FSTL1 has been reported to exhibit both pro- and anti-inflammatory actions, with a specific anti-apoptotic and protective effect in cardiac or renal cell injury. Zwijsen et al. (1994) detected several isoforms of FSTL1 with molecular masses of 40 to 48 kD, which differ from the 50- to 55-kD products detected by Tanaka et al. (1998) (OMIM). (PMID: 26386648; PMID: 23723173; PMID: 20176958).