Tested Applications
| Positive WB detected in | HEK-293 cells, U-251 cells, NIH/3T3 cells |
| Positive IHC detected in | human testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27707-1-AP targets FTSJD2 in WB, IHC, IF/ICC, CoIP, ELISA, CUT&Tag applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26722 Product name: Recombinant human FTSJD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 499-835 aa of BC031890 Sequence: SNESHCSLQIKALAKIHAFVQDTTLSEPRQAEIRKECLRLWGIPDQARVAPSSSDPKSKFFELIQGTEIDIFSYKPTLLTSKTLEKIRPVFDYRCMVSGSEQKFLIGLGKSQIYTWDGRQSDRWIKLDLKTELPRDTLLSVEIVHELKGEGKAQRKISAIHILDVLVLNGTDVREQHFNQRIQLAEKFVKAVSKPSRPDMNPIRVKEVYRLEEMEKIFVRLEMKIIKGSSGTPKLSYTGRDDRHFVPMGLYIVRTVNEPWTMGFSKSFKKKFFYNKKTKDSTFDLPADSIAPFHICYYGRLFWEWGDGIRVHDSQKPQDQDKLSKEDVLSFIQMHRA Predict reactive species |
| Full Name | FtsJ methyltransferase domain containing 2 |
| Calculated Molecular Weight | 835 aa, 95 kDa |
| Observed Molecular Weight | 96-100 kDa |
| GenBank Accession Number | BC031890 |
| Gene Symbol | FTSJD2 |
| Gene ID (NCBI) | 23070 |
| RRID | AB_2880949 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N1G2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cap1 2′-O-methylation is catalyzed by cap methyltransferase 1 (CMTR1). CMTR1, also called IFN-stimulated gene 95 kDa protein (ISG95) or FTSJD2, is elevated by a viral infection and required to promote IFN-mediated induction of ISGs and the antiviral response. High CMTR1 expression is associated with poor prognosis in patients with CRC (PMID: 37024465). CMTr1 binds ATP-dependent RNA DHX15 helicase and that this interaction, mediated by the G-patch domain of CMTr1, has an advantageous effect on CMTr1 activity towards highly structured RNA substrates (PMID: 30397098).). Human CMTR1 is a 95 kDa nuclear protein that has multiple domains, consisting of a G-patch domain, a RrmJ/FtsJ methyltransferase domain, a non-functional cap guanylyltransferase-like domain and a WW domain (PMID: 30312682).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FTSJD2 antibody 27707-1-AP | Download protocol |
| IHC protocol for FTSJD2 antibody 27707-1-AP | Download protocol |
| WB protocol for FTSJD2 antibody 27707-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FTSJD2 (CMTR1/ISG95) polyclonal antibody, catalog number 27707-1-AP, has 1 total citation. The cited publication appeared in Molecular Cell and investigated the ECAS complex that couples transcription elongation and RNA processing, placing the research in the fields of RNA biology and transcriptional regulation. The antibody was used in CUT&Tag and CoIP applications with human and mouse samples.
| Species | Application | Title |
|---|---|---|
