- Featured Product
- KD/KO Validated
FUT8 Monoclonal antibody
FUT8 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
WB, IHC, ELISA
Cat no : 66118-1-Ig
Validation Data Gallery
|Positive WB detected in||MDA-MB-231 cells, MDA-MB-453s cells, MCF-7 cells, Caco-2 cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 7 publications below|
|IHC||See 5 publications below|
The immunogen of 66118-1-Ig is FUT8 Fusion Protein expressed in E. coli.
|Host / Isotype||Mouse / IgG2a|
|Immunogen||FUT8 fusion protein Ag18769|
|Full Name||fucosyltransferase 8 (alpha (1,6) fucosyltransferase)|
|Calculated molecular weight||66 kDa|
|Observed molecular weight||66 kDa|
|GenBank accession number||BC025385|
|Gene ID (NCBI)||2530|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).
|Product Specific Protocols|
|WB protocol for FUT8 antibody 66118-1-Ig||Download protocol|
|IHC protocol for FUT8 antibody 66118-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
A Systems Biology Approach Identifies FUT8 as a Driver of Melanoma Metastasis.
Am J Hum Genet
Biallelic Mutations in FUT8 Cause a Congenital Disorder of Glycosylation with Defective Fucosylation.
Mol Cell Proteomics
Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei.
Am J Cancer Res
Fucosyltransferase 8 modulates receptor tyrosine kinase activation and temozolomide resistance in glioblastoma cells.
Mol Cell Proteomics
Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei
Altered fucosyltransferase expression in the superior temporal gyrus of elderly patients with schizophrenia.