Tested Applications
| Positive WB detected in | MDA-MB-231 cells, MDA-MB-453s cells, MCF-7 cells, Caco-2 cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human lung cancer tissue, mouse cerebellum tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66118-1-Ig targets FUT8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18769 Product name: Recombinant human FUT8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 276-575 aa of BC025385 Sequence: HWSGEVKDKNVQVVEFPIVDSLHPRPPYLPLAVPEDLADRLVRVHGDPAVWWVSQFVKYLIRPQPWLEKEIEEATKKLGFKHPVIGVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQLLARRMQVDKKRVYLATDDPSLLKEAKTKYPNYEFISDNSISWSAGLHNRYTENSLRGVILDIHFLSQADFLVCTFSSQVCRVAYEIMQTLHPDASANFHSLDDIYYFGGQNAHNQIAIYAHQPRTADEIPMEPGDIIGVAGNHWDGYSKGVNRKLGRTGLYPSYKVREKIETVKYPTYPEAEK Predict reactive species |
| Full Name | fucosyltransferase 8 (alpha (1,6) fucosyltransferase) |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC025385 |
| Gene Symbol | FUT8 |
| Gene ID (NCBI) | 2530 |
| RRID | AB_2881517 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BYC5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FUT8 antibody 66118-1-Ig | Download protocol |
| WB protocol for FUT8 antibody 66118-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FUT8 Monoclonal antibody (Cat# 66118-1-Ig) has 20 citations. They appear in journals including Cancer Cell, Nature Communications, Oncogene, Cell Death Discovery, Oncogenesis, American Journal of Human Genetics, Molecular & Cellular Proteomics, Journal of Cell Biology, International Journal of Molecular Sciences, Glycobiology, and others. Research fields span cancer biology (melanoma, breast, ovarian, renal, colorectal, glioblastoma, lung, cholangiocarcinoma), glycosylation/fucosylation, immune checkpoint therapy, viral infection, metabolic reprogramming, and congenital disorders of glycosylation.
| Species | Application | Title |
|---|---|---|
Cancer Cell A Systems Biology Approach Identifies FUT8 as a Driver of Melanoma Metastasis.
| ||
Nat Commun EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses | ||
Phytomedicine Ginsenoside Rg3 improves atezolizumab immune checkpoint therapy in triple-negative breast cancer by targeting FUT8-mediated PD-L1 N-glycosylation.
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Cell Death Discov FUT8 reprograms glycolytic metabolism to promote PKM2 lactylation and drive clear cell renal cell carcinoma progression.
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Oncogene Core fucosylation of NCEH1 by FUT8 promotes progression of high-grade serous ovarian cancer by driving tumor-associated macrophage M2 polarization.
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Int J Biol Macromol Genome-wide CRISPR screen identifies ALG5, ALG6, NF2, and FUT8 as key host proteins involved in transmissible gastroenteritis virus infection.
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