Filter: AllWBIHCIF

WB analysis of pig brain using 60230-1-Ig (same clone as 60230-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 60230-1-Ig (same clone as 60230-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 60230-1-Ig (same clone as 60230-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.
WB analysis of fetal human brain using 60230-1-Ig (same clone as 60230-1-PBS)
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 60230-1-Ig (same clone as 60230-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 60230-1-Ig (same clone as 60230-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60230-1-Ig (same clone as 60230-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60230-1-Ig (same clone as 60230-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 60230-1-Ig (same clone as 60230-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 60230-1-Ig (same clone as 60230-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 60230-1-Ig (same clone as 60230-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach using 60230-1-Ig(FUT9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 60230-1-Ig (same clone as 60230-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach using 60230-1-Ig(FUT9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.
IF Staining of human breast cancer using 60230-1-Ig (same clone as 60230-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FUT9 antibody (60230-1-Ig, Clone: 5D4G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

FUT9 Monoclonal antibody, PBS Only

FUT9 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 60230-1-PBS

Clone No.5D4G6

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Alpha (1,3) fucosyltransferase, Fuc TIX, Fucosyltransferase 9, Fucosyltransferase IX, FucT IX, FUT9

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60230-1-PBS targets FUT9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity	human, pig, mouse, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag8298 Product name: Recombinant human FUT9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 32-359 aa of BC036101 Sequence: PTNSWIFSPMESASSVLKMKNFFSTKTDYFNETTILVWVWPFGQTFDLTSCQAMFNIQGCHLTTDRSLYNKSHAVLIHHRDISWDLTNLPQQARPPFQKWIWMNLESPTHTPQKSGIEHLFNLTLTYRRDSDIQVPYGFLTVSTNPFVFEVPSKEKLVCWVVSNWNPEHARVKYYNELSKSIEIHTYGQAFGEYVNDKNLIPTISACKFYLSFENSIHKDYITEKLYNAFLAGSVPVVLGPSRENYENYIPADSFIHVEDYNSPSELAKYLKEVDKNNKLYLSYFNWRKDFTVNLPRFWESHACLACDHVKRHQEYKSVGNLEKWFWN Predict reactive species
Full Name	fucosyltransferase 9 (alpha (1,3) fucosyltransferase)
Calculated Molecular Weight	359 aa, 42 kDa
Observed Molecular Weight	44-46 kDa
GenBank Accession Number	BC036101
Gene Symbol	FUT9
Gene ID (NCBI)	10690
RRID	AB_11183046
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9Y231
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes.