Tested Applications
| Positive WB detected in | pig brain tissue, rat brain tissue, mouse brain tissue, fetal human brain tissue |
| Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60230-1-Ig targets FUT9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8298 Product name: Recombinant human FUT9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 32-359 aa of BC036101 Sequence: PTNSWIFSPMESASSVLKMKNFFSTKTDYFNETTILVWVWPFGQTFDLTSCQAMFNIQGCHLTTDRSLYNKSHAVLIHHRDISWDLTNLPQQARPPFQKWIWMNLESPTHTPQKSGIEHLFNLTLTYRRDSDIQVPYGFLTVSTNPFVFEVPSKEKLVCWVVSNWNPEHARVKYYNELSKSIEIHTYGQAFGEYVNDKNLIPTISACKFYLSFENSIHKDYITEKLYNAFLAGSVPVVLGPSRENYENYIPADSFIHVEDYNSPSELAKYLKEVDKNNKLYLSYFNWRKDFTVNLPRFWESHACLACDHVKRHQEYKSVGNLEKWFWN Predict reactive species |
| Full Name | fucosyltransferase 9 (alpha (1,3) fucosyltransferase) |
| Calculated Molecular Weight | 359 aa, 42 kDa |
| Observed Molecular Weight | 44-46 kDa |
| GenBank Accession Number | BC036101 |
| Gene Symbol | FUT9 |
| Gene ID (NCBI) | 10690 |
| RRID | AB_11183046 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y231 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| IHC protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| WB protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FUT9 antibody (Cat# 60230-1-Ig) has 3 total citations published in Scientific Reports, Cancers (Basel), and Acta Biochimica et Biophysica Sinica. These studies span colorectal cancer biomarker discovery, colon cancer stem cell programming, and esophageal squamous cell carcinoma metastasis, with applications in Western blot and immunohistochemistry across human and mouse species.
| Species | Application | Title |
|---|---|---|
Sci Rep Integrative multi-omics analysis identified FUT9 and MS4A3 as novel immune-phenotype and prognosis biomarkers for colorectal cancer and analyze the role of FUT9 in oncoimmunology.
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Cancers (Basel) FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like State.
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Acta Biochim Biophys Sin (Shanghai) ELF4 contributes to esophageal squamous cell carcinoma growth and metastasis by augmenting cancer stemness via FUT9
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