CoraLite® Plus 488-conjugated FUT9 Monoclonal antibody

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  IF Staining of MCF-7 using CL488-60230

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 FUT9 antibody (CL488-60230, Clone: 5D4G6 ) at dilution of 1:200.

• Featured Product
• KD/KO Validated

FUT9 Monoclonal Antibody for IF/ICC

Cat No. CL488-60230

Clone No.5D4G6

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse, rat

Applications

IF/ICC

Synonyms

Alpha (1,3) fucosyltransferase, Fuc TIX, Fucosyltransferase 9, Fucosyltransferase IX, FucT IX, FUT9

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60230 targets FUT9 in IF/ICC applications and shows reactivity with human, pig, mouse, rat samples.

• Tested Reactivity: human, pig, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8298

  Product name: Recombinant human FUT9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 32-359 aa of BC036101

  Sequence: PTNSWIFSPMESASSVLKMKNFFSTKTDYFNETTILVWVWPFGQTFDLTSCQAMFNIQGCHLTTDRSLYNKSHAVLIHHRDISWDLTNLPQQARPPFQKWIWMNLESPTHTPQKSGIEHLFNLTLTYRRDSDIQVPYGFLTVSTNPFVFEVPSKEKLVCWVVSNWNPEHARVKYYNELSKSIEIHTYGQAFGEYVNDKNLIPTISACKFYLSFENSIHKDYITEKLYNAFLAGSVPVVLGPSRENYENYIPADSFIHVEDYNSPSELAKYLKEVDKNNKLYLSYFNWRKDFTVNLPRFWESHACLACDHVKRHQEYKSVGNLEKWFWN

• Full Name: fucosyltransferase 9 (alpha (1,3) fucosyltransferase)
• Calculated Molecular Weight: 359 aa, 42 kDa
• Observed Molecular Weight: 44-46 kDa
• GenBank Accession Number: BC036101
• Gene Symbol: FUT9
• Gene ID (NCBI): 10690
• RRID: AB_3084143
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y231
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes.