FXYD2 Polyclonal antibody

FXYD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11198-1-AP

Synonyms

ATP1C, ATP1G1, FXYD2, HOMG2, Sodium pump gamma chain



Tested Applications

Positive WB detected inhuman kidney tissue, human liver tissue, human skeletal muscle tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11198-1-AP targets FXYD2 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FXYD2 fusion protein Ag1676
Full Name FXYD domain containing ion transport regulator 2
Calculated Molecular Weight 7 kDa
Observed Molecular Weight 7 kDa
GenBank Accession NumberBC013289
Gene Symbol FXYD2
Gene ID (NCBI) 486
RRIDAB_2108309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FXYD2 (FXYD domain-containing ion transport regulator 2), also known as the gamma-subunit of the NaK-ATPase, belongs to the FXYD family which has been proposed to be the regulators of Na, K-ATPase function by lowering affinities of the system for potassium and sodium. The expression of FXYD2 is most abundant in kidney, while it is also detected in several other tissues like placenta, pancreas, and dorsal root ganglia (DRGs). Three splice variants of FXYD2 have been reported in mouse kidney, namely FXYD2 γa, -γb,and -γc. FXYD2 γa has been identified as a pancreatic beta cell-specific biomarker. This antibody can recognize all three isoforms of FXYD2.

Protocols

Product Specific Protocols
WB protocol for FXYD2 antibody 11198-1-APDownload protocol
IHC protocol for FXYD2 antibody 11198-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell

Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair.

Authors - Lu Catherine P CP
mouseIHC

Front Cell Dev Biol

A Na+/K+ ATPase Pump Regulates Chondrocyte Differentiation and Bone Length Variation in Mice.

Authors - Marta Marchini
ratIHC

Nutrients

Effect of Dapagliflozin and Magnesium Supplementation on Renal Magnesium Handling and Magnesium Homeostasis in Metabolic Syndrome.

Authors - Hwee-Yeong Ng
mouseIF

Cell Rep

Tumor vessel co-option probed by single-cell analysis.

Authors - Laure-Anne Teuwen
humanWB,IF

J Immunol Res

Downregulation of FXYD2 Is Associated with Poor Prognosis and Increased Regulatory T Cell Infiltration in Clear Cell Renal Cell Carcinoma

Authors - Zedan Zhang
mouseWB

Brain Res Bull

Microglial SIX2 suppresses lipopolysaccharide (LPS)-induced neuroinflammation by up-regulating FXYD2 expression

Authors - Xia-Yin Cao