FXYD2 Polyclonal antibody

FXYD2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11198-1-AP

Synonyms

ATP1C, ATP1G1, FXYD2, HOMG2, Sodium pump gamma chain

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Validation Data Gallery

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  WB analysis of human kidney using 11198-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11198-1-AP (FXYD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11198-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11198-1-AP (FXYD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11198-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11198-1-AP (FXYD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11198-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11198-1-AP (FXYD2 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: human kidney tissue, human liver tissue, human skeletal muscle tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

11198-1-AP targets FXYD2 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FXYD2 fusion protein Ag1676
• Full Name: FXYD domain containing ion transport regulator 2
• Calculated Molecular Weight: 7 kDa
• Observed Molecular Weight: 7 kDa
• GenBank Accession Number: BC013289
• Gene Symbol: FXYD2
• Gene ID (NCBI): 486
• RRID: AB_2108309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FXYD2 (FXYD domain-containing ion transport regulator 2), also known as the gamma-subunit of the NaK-ATPase, belongs to the FXYD family which has been proposed to be the regulators of Na, K-ATPase function by lowering affinities of the system for potassium and sodium. The expression of FXYD2 is most abundant in kidney, while it is also detected in several other tissues like placenta, pancreas, and dorsal root ganglia (DRGs). Three splice variants of FXYD2 have been reported in mouse kidney, namely FXYD2 γa, -γb,and -γc. FXYD2 γa has been identified as a pancreatic beta cell-specific biomarker. This antibody can recognize all three isoforms of FXYD2.

Publications

Species	Application	Title
mouse	IHC	Cell; Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair.; Authors - Lu Catherine P CP
mouse	IHC	Front Cell Dev Biol; A Na+/K+ ATPase Pump Regulates Chondrocyte Differentiation and Bone Length Variation in Mice.; Authors - Marta Marchini
rat	IHC	Nutrients; Effect of Dapagliflozin and Magnesium Supplementation on Renal Magnesium Handling and Magnesium Homeostasis in Metabolic Syndrome.; Authors - Hwee-Yeong Ng
mouse	IF	Cell Rep; Tumor vessel co-option probed by single-cell analysis.; Authors - Laure-Anne Teuwen
human	WB,IF	J Immunol Res; Downregulation of FXYD2 Is Associated with Poor Prognosis and Increased Regulatory T Cell Infiltration in Clear Cell Renal Cell Carcinoma; Authors - Zedan Zhang