Factor IX/PTC Recombinant antibody, PBS Only

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  WB analysis of human plasma using 84533-4-RR (same clone as 84533-4-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 84533-4-RR (Coagulation factor IX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84533-4-PBS in a different storage buffer formulation.

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  IHC staining of Human Hepatocellular cancer using 84533-4-RR (same clone as 84533-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84533-4-RR (Factor IX/PTC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84533-4-PBS in a different storage buffer formulation.

Factor IX/PTC Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84533-4-PBS

Clone No.241742G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain

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Product Information

84533-4-PBS targets Factor IX/PTC in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Factor IX/PTC fusion protein Eg1045
• Full Name: coagulation factor IX
• Calculated Molecular Weight: 52kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: NM_000133.4
• Gene Symbol: F9
• Gene ID (NCBI): 2158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P00740-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The F9 gene encodes coagulation factor IX, which circulates as an inactive zymogen until proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Its role in the blood coagulation cascade is to activate factor X (F10) through interactions with calcium, membrane phospholipids, and factor VIII (F8). Factor IX and factor X both consist of 2 polypeptide chains referred to as the L (light) and H (heavy) chains. The H chain bears a structural resemblance to the polypeptide chain of the pancreatic serine protease trypsin (PRSS1). The L chain is covalently linked to the H chain by a single disulfide bond. Native Factor IX MW is around 72kDa(PMID:827443; 12496253), and it can be cleaved into different fragments.