Factor IX/PTC Recombinant antibody

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  WB analysis of human plasma using 84533-4-RR

  human plasma were subjected to SDS PAGE followed by western blot with 84533-4-RR (Coagulation factor IX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of Human Hepatocellular cancer using 84533-4-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84533-4-RR (Factor IX/PTC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Factor IX/PTC Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 84533-4-RR

Clone No.241742G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain

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Tested Applications

• Positive WB detected in: human plasma
• Positive IHC detected in: Human Hepatocellular cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84533-4-RR targets Factor IX/PTC in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Factor IX/PTC fusion protein Eg1045
• Full Name: coagulation factor IX
• Calculated Molecular Weight: 52kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: NM_000133.4
• Gene Symbol: F9
• Gene ID (NCBI): 2158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P00740-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The F9 gene encodes coagulation factor IX, which circulates as an inactive zymogen until proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Its role in the blood coagulation cascade is to activate factor X (F10) through interactions with calcium, membrane phospholipids, and factor VIII (F8). Factor IX and factor X both consist of 2 polypeptide chains referred to as the L (light) and H (heavy) chains. The H chain bears a structural resemblance to the polypeptide chain of the pancreatic serine protease trypsin (PRSS1). The L chain is covalently linked to the H chain by a single disulfide bond. Native Factor IX MW is around 72kDa(PMID:827443; 12496253), and it can be cleaved into different fragments.