CoraLite® Plus 488-conjugated Factor X Monoclonal antibody

Factor X Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D3F11

Cat no : CL488-66753

Synonyms

coagulation factor X, F10, FX, FXA, Stuart factor, Stuart Prower factor

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Validation Data Gallery

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  IF Staining of human liver using CL488-66753

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite® Plus 488 Factor X antibody (CL488-66753, Clone: 3D3F11 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human liver tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66753 targets Factor X in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Factor X fusion protein Ag11664
• Full Name: coagulation factor X
• Calculated Molecular Weight: 488 aa, 55 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC046125
• Gene Symbol: F10
• Gene ID (NCBI): 2159
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coagulation factor X (F10) is a vitamin K-dependent glycoprotein that participates in the middle phase of the blood coagulation cascade (PMID: 1931959; 1313796). It is synthesized as a single-chain precursor in the liver where it undergoes a number of covalent processing steps before secretion into the blood as a two-chain molecule linked by a disulfide bond (PMID: 1313796). The light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. Factor X is activated into factor Xa, by both the extrinsic and intrinsic pathway. The activated factor then converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.