Ferroptosis Essentials Antibody Kit
Cat no : PK30002
The Ferroptosis Essentials Antibody Kit provides a cost-effective tool for studying key proteins involved in the ferroptosis pathway. Perfect for researchers starting a new project, screening multiple prospective targets or those who simply require less volume.
The Ferroptosis Essentials Antibody Kit contains antibodies against 5 key protein targets playing critical roles in the ferroptotic cell death pathway.
|Antigen||Catalog No.||Host, clonality||Tested Reactivity||Applications||Volume|
|GPX4||67763-1-Ig||Mouse monoclonal||H, M, R, Rb, Pg||WB, IHC, IF, CoIP, ELISA||20 uL|
|SLC7A11/xCT||26864-1-AP||Rabbit polyclonal||H, M, R||WB, RIP, IHC, IF, ELISA||20 uL|
|KEAP1||80744-1-RR||Rabbit monoclonal||H, M, R||WB, IHC, IF, ELISA||20 uL|
|NRF2||80593-1-RR||Rabbit monoclonal||H, M||WB, IP, IF, ELISA||20 uL|
|ACSL4||22401-1-AP||Rabbit polyclonal||H, M||WB, IP, IHC, IF, ELISA||20 uL|
Also see our 'Ferroptosis Expanded Antibody Kit' on the following page https://www.ptglab.com/products/Ferroptosis-Expanded-Antibody-Kit-PK30003.htm
Store at -20°C. Stable for one year from the date of receipt.
Ferroptosis is an iron-dependent form of regulated cell death characterized by an increase in intracellular reactive oxygen species (ROS) levels. The peroxidase GPX4, whose activity relies on glutathione (GSH) biosynthesis, is a key regulator of the ferroptosis pathway. GPX4 utilizes GSH as a cofactor to reduce intracellular lipid peroxides. Inactivation of GPX4 caused by intracellular GSH depletion leads to ROS accumulation, thereby triggering ferroptosis. Ferroptosis can also be regulated by the cell surface cysteine-glutamate antiporter (system xc-) consisting of SLC7A11 and SLC3A2 in conjunction with the glutathione metabolic pathway. Inhibition of system xc- prevents glutathione synthesis by inhibiting cysteine absorption, leading to oxidative stress and impairment of GPX4 activity, which in turn promotes ferroptosis. The KEAP1-NRF2 pathway has been shown to play a protective role against ferroptosis in multiple disease models. ACSL4, an enzyme mediating fatty acid metabolism acts as a key driver and biomarker of ferroptosis under specific conditions.
Click here to view our standard protocols for various applications including WB, IP, IHC, IF, FC, and ELISA.
Cited in Article as
PK30002, Ferroptosis Essentials Antibody Kit, Proteintech, IL, USA