NRF2, NFE2L2 Recombinant antibody

NRF2, NFE2L2 Recombinant Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1I21

Cat no : 80593-1-RR

Synonyms

HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2

---

Validation Data Gallery

• 

  WB analysis using 80593-1-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80593-1-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80593-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80593-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 80593-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NRF2, NFE2L2 antibody (80593-1-RR, Clone: 1I21 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 80593-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRF2, NFE2L2 antibody (80593-1-RR, Clone: 1I21 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, NIH/3T3 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

80593-1-RR targets NRF2, NFE2L2 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NRF2, NFE2L2 fusion protein Ag9489
• Full Name: nuclear factor (erythroid-derived 2)-like 2
• Calculated molecular weight: 605 aa, 68 kDa
• Observed molecular weight: 110 kDa
• GenBank accession number: BC011558
• Gene symbol: NFE2L2
• Gene ID (NCBI): 4780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Publications

Species	Application	Title
human,mouse	WB	Br J Pharmacol; Leonurine hydrochloride alleviates ferroptosis in cisplatin-induced acute kidney injury by activating the Nrf2 signaling pathway.; Authors - Jianqiang Hu ; KO Validated