• Featured Product
  • KD/KO Validated

NRF2, NFE2L2 Recombinant antibody

NRF2, NFE2L2 Recombinant Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB, IP, IHC, IF, FC, ChIP, ELISA

Conjugate

Unconjugated

CloneNo.

1I21

Cat no : 80593-1-RR

Synonyms

HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2



Tested Applications

Positive WB detected inMG132 treated HEK-293 cells, MG132 treated HepG2 cells, MG132 treated HeLa cells, MG132 treated NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMG132 treated HepG2 cells, HepG2 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:300-1:1200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

80593-1-RR targets NRF2, NFE2L2 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NRF2, NFE2L2 fusion protein Ag9489
Full Name nuclear factor (erythroid-derived 2)-like 2
Calculated molecular weight 605 aa, 68 kDa
Observed molecular weight110 kDa
GenBank accession numberBC011558
Gene symbol NFE2L2
Gene ID (NCBI) 4780
RRIDAB_2918904
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Protocols

Product Specific Protocols
WB protocol for NRF2, NFE2L2 antibody 80593-1-RRDownload protocol
IHC protocol for NRF2, NFE2L2 antibody 80593-1-RRDownload protocol
IF protocol for NRF2, NFE2L2 antibody 80593-1-RRDownload protocol
IP protocol for NRF2, NFE2L2 antibody 80593-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Host Microbe

Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice

Authors - Yunong Zeng
humanWB

J Med Virol

SARS-CoV-2 nucleocapsid protein enhances the level of mitochondrial reactive oxygen species

Authors - Haiyun Yu
human,mouseWB

Br J Pharmacol

Leonurine hydrochloride alleviates ferroptosis in cisplatin-induced acute kidney injury by activating the Nrf2 signaling pathway.

Authors - Jianqiang Hu
  • KO Validated
humanWB

Antioxidants (Basel)

Glycolysis Aids in Human Lens Epithelial Cells' Adaptation to Hypoxia

Authors - Yuxin Huang
humanWB

Mol Med

β-aminoisobutyrics acid, a metabolite of BCAA, activates the AMPK/Nrf-2 pathway to prevent ferroptosis and ameliorates lung ischemia-reperfusion injury

Authors - Ziyue Zhang
mouseWB

Chem Biol Interact

Pectolinarigenin attenuates hepatic ischemia/reperfusion injury via activation of the PI3K/AKT/Nrf2 signaling pathway

Authors - Hao Li