NRF2, NFE2L2 Recombinant antibody

NRF2, NFE2L2 Recombinant Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1I21

Cat no : 80593-1-RR

Synonyms

HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, NIH/3T3 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

80593-1-RR targets NRF2, NFE2L2 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NRF2, NFE2L2 fusion protein Ag9489
Full Name nuclear factor (erythroid-derived 2)-like 2
Calculated molecular weight 605 aa, 68 kDa
Observed molecular weight110 kDa
GenBank accession numberBC011558
Gene symbol NFE2L2
Gene ID (NCBI) 4780
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Protocols

Product Specific Protocols
WB protocol for NRF2, NFE2L2 antibody 80593-1-RRDownload protocol
IF protocol for NRF2, NFE2L2 antibody 80593-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Br J Pharmacol

Leonurine hydrochloride alleviates ferroptosis in cisplatin-induced acute kidney injury by activating the Nrf2 signaling pathway.

Authors - Jianqiang Hu
  • KO Validated