Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human endometrial cancer tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29425-1-AP targets Fibrillin 1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30283 Product name: Recombinant human FBN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 367-462 aa of BC146854 Sequence: SPGVTVAPEMCPIRATEDFNKLCSVPMVIPGRPEYPPPPLGPIPPVLPVPPGFPPGPQIPVPRPPVEYLYPSREPPRVLPVNVTDYCQLVRYLCQN Predict reactive species |
| Full Name | fibrillin 1 |
| Observed Molecular Weight | 350 kDa |
| GenBank Accession Number | BC146854 |
| Gene Symbol | Fibrillin 1 |
| Gene ID (NCBI) | 2200 |
| RRID | AB_3086131 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35555 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fibrillin 1 is a 350 kDa large extracellular matrix glycoprotein that formed thread-like microfibril filaments, which provided structural support for tissues, formed elastic fibers in the skin and vessels, regulated the availability of the transforming growth factor beta (TGF-β) protein, and modulated the microenvironment. Fibrillin 1 contains a large number of cysteine-rich epidermal growth factor (EGF)- like domains, most capable of binding calcium ions (cbEGF-like), and a few motifs homologous to the binding protein for transforming growth factor beta (TGFβ1-BP)- like module (PMID: 29220879, PMID: 35234370).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Fibrillin 1 antibody 29425-1-AP | Download protocol |
| IHC protocol for Fibrillin 1 antibody 29425-1-AP | Download protocol |
| WB protocol for Fibrillin 1 antibody 29425-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Fibrillin 1 Polyclonal antibody (Cat# 29425-1-AP) has 2 citations. One appeared in RSC Advances (2025), studying ulcerative colitis biomarkers via IHC in human samples. The other was published in Cells (2024), investigating ADAR1's role in smooth muscle homeostasis and vascular integrity using WB and CoIP in mouse models. The associated research fields span gastroenterology (inflammatory bowel disease) and cardiovascular/vascular biology.
| Species | Application | Title |
|---|---|---|
RSC Adv Quantitative proteomic and glycoproteomic analysis identifies CLCA1, FBN1, and FGB as potential biomarkers for ulcerative colitis. | ||
