Fibrinogen Alpha Chain Recombinant monoclonal antibody

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• Fibrinogen Alpha Chain Recombinant monoclonal antibody (86913-6-RR)

Fibrinogen Alpha Chain Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86913-6-RR

Clone No.260891B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

FGA, Fib2, Fibrinopeptide A

• 

  WB analysis using 86913-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86913-6-RR (FGA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 86913-6-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86913-6-RR (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 86913-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86913-6-RR (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human placenta tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86913-6-RR targets Fibrinogen Alpha Chain in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag33674

  Product name: Recombinant human FGA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 20-218 aa of BC020764

  Sequence: ADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSGEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVTANNLLVARVTTEETPHLKARAIKWQMRPEVKPIMKEHIAPREAMLNLALSEVSTLLLWGSLPCPPRLS*

• Full Name: fibrinogen alpha chain
• Calculated Molecular Weight: 866 aa, 95 kDa
• Observed Molecular Weight: 95kDa, 70 kDa
• GenBank Accession Number: BC020764
• Gene Symbol: Fibrinogen Alpha Chain
• Gene ID (NCBI): 2243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02671
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGA is the alpha chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide A is cleaved from FGA by thrombin.