Fibronectin Recombinant monoclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• Fibronectin Recombinant monoclonal antibody, PBS Only (80911-4-PBS)

Fibronectin Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 80911-4-PBS

Clone No.251989E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FN1, Anastellin, CIG, Cold insoluble globulin, Cold-insoluble globulin

• 

  WB analysis using 80911-4-RR (same clone as 80911-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80911-4-RR (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80911-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 80911-4-RR (same clone as 80911-4-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80911-4-RR (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80911-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat plasma using 80911-4-RR (same clone as 80911-4-PBS)

  rat plasma were subjected to SDS PAGE followed by western blot with 80911-4-RR (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80911-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NIH/3T3 using 80911-4-RR (same clone as 80911-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (80911-4-RR, Clone: 251989E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80911-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Product Information

80911-4-PBS targets Fibronectin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5117

  Product name: Recombinant Human Fibronectin protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 607-1265 aa of NM_212482.4

  Sequence: SSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIP

• Full Name: fibronectin 1
• Calculated Molecular Weight: 272 kDa
• Observed Molecular Weight: 220-275 kDa
• GenBank Accession Number: NM_212482.4
• Gene Symbol: Fibronectin
• Gene ID (NCBI): 2335
• RRID: AB_3743358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02751-15
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fibronectin (fibronectin) is a kind of macromolecular glycoprotein widely existing in extracellular matrix (ECM), plasma and various cell surfaces, which plays a central role in key biological processes such as cell adhesion, migration, proliferation, differentiation, tissue repair and embryo development. Fibronectin is usually a dimer structure, which consists of two subunits connected by disulfide bonds. There are two main forms of Fibronectin: plasma Fibronectin(pFN) is synthesized by liver cells and exists in the blood, which is involved in the processes of coagulation and wound repair. Cellular Fibronectin(cFN) is synthesized by fibroblasts and other cells, deposited in extracellular matrix, and participates in the interaction between cells and matrix. Its molecular weight ranges from 220 to 275 kDa.