Fibronectin Recombinant monoclonal antibody

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• Fibronectin Recombinant monoclonal antibody (80911-4-RR)

Fibronectin Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 80911-4-RR

Clone No.251989E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

FN1, Anastellin, CIG, Cold insoluble globulin, Cold-insoluble globulin

• 

  WB analysis using 80911-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80911-4-RR (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 80911-4-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80911-4-RR (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat plasma using 80911-4-RR

  rat plasma were subjected to SDS PAGE followed by western blot with 80911-4-RR (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NIH/3T3 using 80911-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (80911-4-RR, Clone: 251989E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HepG2 cells, rat plasma, mouse uterus tissue
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80911-4-RR targets Fibronectin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5117

  Product name: Recombinant Human Fibronectin protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 607-1265 aa of NM_212482.4

  Sequence: SSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIP

• Full Name: fibronectin 1
• Calculated Molecular Weight: 272 kDa
• Observed Molecular Weight: 220-275 kDa
• GenBank Accession Number: NM_212482.4
• Gene Symbol: Fibronectin
• Gene ID (NCBI): 2335
• RRID: AB_3743358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02751-15
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibronectin (fibronectin) is a kind of macromolecular glycoprotein widely existing in extracellular matrix (ECM), plasma and various cell surfaces, which plays a central role in key biological processes such as cell adhesion, migration, proliferation, differentiation, tissue repair and embryo development. Fibronectin is usually a dimer structure, which consists of two subunits connected by disulfide bonds. There are two main forms of Fibronectin: plasma Fibronectin(pFN) is synthesized by liver cells and exists in the blood, which is involved in the processes of coagulation and wound repair. Cellular Fibronectin(cFN) is synthesized by fibroblasts and other cells, deposited in extracellular matrix, and participates in the interaction between cells and matrix. Its molecular weight ranges from 220 to 275 kDa.