Flightless I Monoclonal antibody, PBS Only

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  WB analysis using 67039-1-Ig (same clone as 67039-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67039-1-Ig (FLII antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67039-1-Ig (same clone as 67039-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67039-1-Ig (FLII antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67039-1-Ig (same clone as 67039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67039-1-Ig (FLII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67039-1-Ig (same clone as 67039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67039-1-Ig (FLII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

Flightless I Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67039-1-PBS

Clone No.2F9C8

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Synonyms

FLI, Fli1, Flightless I, FLII, FLIL, Protein flightless 1 homolog

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Product Information

67039-1-PBS targets Flightless I in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Flightless I fusion protein Ag26865
• Full Name: flightless I homolog (Drosophila)
• Calculated Molecular Weight: 1269 aa, 145 kDa
• Observed Molecular Weight: 145-150 kDa
• GenBank Accession Number: BC025300
• Gene Symbol: Flightless I
• Gene ID (NCBI): 2314
• RRID: AB_2882353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13045
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).