Flotillin 2 Monoclonal antibody

Flotillin 2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1E11G12

Cat no : 66881-1-Ig

Synonyms

ECS 1, ECS1, Epidermal surface antigen, ESA, ESA1, FLOT2, flotillin 2, M17S1



Tested Applications

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, A549 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66881-1-Ig targets Flotillin 2 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Flotillin 2 fusion protein Ag28308
Full Name flotillin 2
Calculated molecular weight 379 aa, 42 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC017292
Gene symbol FLOT2
Gene ID (NCBI) 2319
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Protocols

Product Specific Protocols
WB protocol for Flotillin 2 antibody 66881-1-IgDownload protocol
IHC protocol for Flotillin 2 antibody 66881-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols