|Positive WB detected in||pig brain tissue, A431 cells, HeLa cells, HEK-293 cells, A549 cells, NIH/3T3 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66881-1-Ig targets Flotillin 2 in WB, IHC, CoIP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.
|Tested Reactivity||Human, Mouse, Rat, Pig, Rabbit|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Flotillin 2 fusion protein Ag28308|
|Full Name||flotillin 2|
|Calculated molecular weight||379 aa, 42 kDa|
|Observed molecular weight||45 kDa|
|GenBank accession number||BC017292|
|Gene ID (NCBI)||2319|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.
J Dermatol Sci
MicroRNA-486-3p promotes the proliferation and metastasis of cutaneous squamous cell carcinoma by suppressing flotillin-2.
Interaction of Clostridium perfringens Epsilon Toxin with the Plasma Membrane: The Role of Amino Acids Y42, Y43 and H162
Int J Oncol
Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2
Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guorong (Verified Customer) (03-22-2022)
The band marked by the red dot may be the target