Flotillin 2 Monoclonal antibody

Flotillin 2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit and More (1)

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1E11G12

Cat no : 66881-1-Ig

Synonyms

ECS 1, ECS1, Epidermal surface antigen, ESA, ESA1, FLOT2, flotillin 2, M17S1



Tested Applications

Positive WB detected inHeLa cells, pig brain tissue, A431 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, HEK-293 cells, A549 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66881-1-Ig targets Flotillin 2 in WB, IHC, CoIP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Flotillin 2 fusion protein Ag28308
Full Name flotillin 2
Calculated molecular weight 379 aa, 42 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC017292
Gene symbol FLOT2
Gene ID (NCBI) 2319
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Protocols

Product Specific Protocols
WB protocol for Flotillin 2 antibody 66881-1-IgDownload protocol
IHC protocol for Flotillin 2 antibody 66881-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Dermatol Sci

MicroRNA-486-3p promotes the proliferation and metastasis of cutaneous squamous cell carcinoma by suppressing flotillin-2.

Authors - Xiangzhi Li
humanWB

Toxins (Basel)

Interaction of Clostridium perfringens Epsilon Toxin with the Plasma Membrane: The Role of Amino Acids Y42, Y43 and H162

Authors - Skye Marshall
humanWB

Int J Oncol

Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2

Authors - Cheng Qian
humanWB,CoIP

Nat Commun

Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo

Authors - Jasjot Singh

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Guorong (Verified Customer) (03-22-2022)

The band marked by the red dot may be the target

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: ARPE-19
Flotillin 2 Antibody Western Blot validation (1:2500 dilution) in ARPE-19 (Cat no:66881-1-Ig)