Tested Applications
| Positive WB detected in | mouse eye tissue, rat eye tissue, mouse heart tissue, rat heart tissue, human skeletal muscle tissue, human heart tissue, human adipose tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87975-1-RR targets Frizzled 4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg5015 Product name: Recombinant human FZD4 protein Source: mammalian cells-derived, pHZ-KIsec-C-mFc Tag: C-mFc Domain: 37-180 aa of BC114527 Sequence: FGDEEERRCDPIRISMCQNLGYNVTKMPNLVGHELQTDAELQLTTFTPLIQYGCSSQLQFFLCSVYVPMCTEKINIPIGPCGGMCLSVKRRCEPVLKEFGFAWPESLNCSKFPPQNDHNHMCMEGPGDEEVPLPHKTPIQPGEE Predict reactive species |
| Full Name | frizzled homolog 4 (Drosophila) |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC114527 |
| Gene Symbol | FZD4 |
| Gene ID (NCBI) | 8322 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9ULV1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Frizzled 4, officially known as frizzled class receptor 4 and designated FZD4, is a protein encoded by the FZD4 gene in humans. It is a member of the frizzled (Fz) gene family, which encodes seven-transmembrane domain proteins that primarily function as receptors for Wnt signaling proteins. Frizzled 4 is embedded in the outer membrane of many cell types, where it plays a critical role in transmitting chemical signals from outside the cell to the nucleus. Frizzled 4 has an almost ubiquitous expression pattern and is found in a wide range of human tissues. Mutations in the FZD4 gene are linked to familial exudative vitreoretinopathy (EVR1), an eye disorder that affects the retina and can lead to progressive vision loss.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Frizzled 4 antibody 87975-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
