G6PC Polyclonal antibody

G6PC Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 22169-1-AP

Synonyms

G 6 Pase, G6P, G6Pase, G6Pase alpha, G6PC, G6PT, Glucose 6 phosphatase, Glucose 6 phosphatase alpha, GSD1, GSD1a



Tested Applications

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

22169-1-AP targets G6PC in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen G6PC fusion protein Ag17839
Full Name glucose-6-phosphatase, catalytic subunit
Calculated molecular weight 357 aa, 40 kDa
GenBank accession numberBC130478
Gene symbol G6PC
Gene ID (NCBI) 2538
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).

Protocols

Product Specific Protocols
IHC protocol for G6PC antibody 22169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Mol Metab

Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.

Authors - Ferrol I Rome
mouse

Mol Nutr Food Res

Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice

Authors - Songfeng Yu
mouse

J Genet Genomics

Functionalized Gadofullerene Ameliorates Impaired Glycolipid Metabolism in Type 2 Diabetic Mice.

Authors - Jin Wu
mouse

Endocrinology

Aspirin Suppresses Hepatic Glucagon Signaling Through Decreasing Production of Thromboxane A2

Authors - Yufeng Dai
human

Pharmacol Res

PMID: 29284152

Authors - Jiting Yan
mouse

Front Mol Biosci

Paternal High-Fat Diet Altered Sperm 5'tsRNA-Gly-GCC Is Associated With Enhanced Gluconeogenesis in the Offspring.

Authors - Bin Wang