G6PC Polyclonal antibody

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  IHC staining of human liver using 22169-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22169-1-AP (G6PC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 22169-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22169-1-AP (G6PC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

G6PC Polyclonal Antibody for IHC, ELISA

Cat No. 22169-1-AP

Publications(41)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, ELISA

Synonyms

G 6 Pase, G6P, G6Pase, G6Pase alpha, G6PC, G6PT, Glucose 6 phosphatase, Glucose 6 phosphatase alpha, GSD1, GSD1a

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Tested Applications

• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

22169-1-AP targets G6PC in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: G6PC fusion protein Ag17839
• Full Name: glucose-6-phosphatase, catalytic subunit
• Calculated Molecular Weight: 357 aa, 40 kDa
• GenBank Accession Number: BC130478
• Gene Symbol: G6PC
• Gene ID (NCBI): 2538
• RRID: AB_2879015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35575
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).

Publications

Species	Application	Title
mouse		Mol Metab; Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.; Authors - Ferrol I Rome
mouse		Mol Nutr Food Res; Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice; Authors - Songfeng Yu
mouse		J Genet Genomics; Functionalized Gadofullerene Ameliorates Impaired Glycolipid Metabolism in Type 2 Diabetic Mice.; Authors - Jin Wu
mouse		Endocrinology; Aspirin Suppresses Hepatic Glucagon Signaling Through Decreasing Production of Thromboxane A2; Authors - Yufeng Dai
human		Pharmacol Res; PMID: 29284152; Authors - Jiting Yan
mouse		Front Mol Biosci; Paternal High-Fat Diet Altered Sperm 5'tsRNA-Gly-GCC Is Associated With Enhanced Gluconeogenesis in the Offspring.; Authors - Bin Wang