G6PD Polyclonal antibody

• Home
• Products
• Primary Antibodies
• G6PD Polyclonal antibody (25413-1-AP)

• Featured Product
• KD/KO Validated

G6PD Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 25413-1-AP Copied!

Publications (77)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

EC:1.1.1.49, G6P, G6PD1, Glucose-6-phosphate 1-dehydrogenase

• 

  WB analysis using 25413-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 25413-1-AP

  WB result of G6PD antibody (25413-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G6PD transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 25413-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 25413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 25413-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 25413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human hepatocellular carcinoma using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma slide using 25413-1-AP (G6PD antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 25413-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using G6PD antibody (25413-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 25413-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug G6PD Polyclonal antibody (25413-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for G6PD in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human hepatocellular carcinoma, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25413-1-AP targets G6PD in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, monkey, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21862

  Product name: Recombinant human G6PD protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-515 aa of BC000337

  Sequence: MAEQVALSRTQVCGILREELFQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAVTKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL

• Full Name: glucose-6-phosphate dehydrogenase
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC000337
• Gene Symbol: G6PD
• Gene ID (NCBI): 2539
• RRID: AB_2880066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11413
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

Publications

What published studies show

The G6PD Polyclonal antibody (catalog number 25413-1-AP) has 77 citations. These appear in high-impact journals including Nature Nanotechnology, Nature Communications, Science Advances, Cell Death & Disease, FASEB Journal, Journal of Biological Chemistry, EMBO Molecular Medicine, and Oncotarget. The research fields span cancer metabolism and the pentose phosphate pathway, ferroptosis, redox homeostasis, drug resistance, and hepatocellular carcinoma, with applications mainly in Western blot, IHC, and IF.

Automatically generated from the 77 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Nanotechnol; Nanoparticles for inducing Gaucher disease-like damage in cancer cells; Authors - Chunyan Yue
		Transl Neurodegener; Transaldolase 1 contributes to pentose phosphate pathway disruption and synaptic dysfunction in Alzheimer's disease.; Authors - Xiaoyu Hu
human	WB,IP	Nat Commun; Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.; Authors - Xiaoyu Ma
human	WB	Nat Commun; Proteogenomics of clear cell renal cell carcinoma response to tyrosine kinase inhibitor; Authors - Hailiang Zhang
human	IF	J Adv Res; Synergistic regulation of SLC7A11 and glucose-6-phosphate dehydrogenase in redox homeostasis governs decidualization: a mechanistic insight into adenomyosis-related infertility.; Authors - Yi Zhang
		Mol Cell; LZTFL1 rewires NADPH-glutathione metabolism to amplify ferroptosis.; Authors - Xiangfei Xue