GABARAPL1 Antibody

GABARAPL1 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 18721-1-AP

Synonyms

APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1, GEC 1, GEC1



Tested Applications

Positive WB detected inmouse kidney tissue, human heart tissue, human spleen tissue, mouse brain tissue, mouse liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

18721-1-AP targets GABARAPL1 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GABA(A) receptor-associated protein like 1
Calculated molecular weight 14 kDa
Observed molecular weight 17 kDa
GenBank accession number NM_031412
Gene symbol GABARAPL1
Gene ID (NCBI) 23710
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It’s also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. And at very low levels in thymus and small intestine.The antibody is specific to GABARAPL1, and it doesn’t recognize the recombinant GABARAP and GABARAPL2.

Protocols

Product Specific Protocols
WB protocol for GABARAPL1 antibody 18721-1-APDownload protocol
IHC protocol for GABARAPL1 antibody 18721-1-APDownload protocol
FC protocol for GABARAPL1 antibody 18721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

GABARAPL1 antibodies: target one protein, get one free!

Authors - Le Grand Jaclyn Nicole JN