CoraLite® Plus 488-conjugated GABPA Recombinant antibody

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  IF Staining of A431 using CL488-84951-4

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 GABPA antibody (CL488-84951-4, Clone: 242578F10 ) at dilution of 1:200, CL594-Phalloidin (red).

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  IF Staining of HeLa using CL488-84951-4

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 GABPA antibody (CL488-84951-4, Clone: 242578F10 ) at dilution of 1:200, CL594-Phalloidin (red).

GABPA Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-84951-4

Clone No.242578F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

242578F10, E4TF1 60, E4TF1A, GA binding protein alpha chain, GA-binding protein alpha chain

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84951-4 targets GABPA in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GABPA fusion protein Ag16191
• Full Name: GA binding protein transcription factor, alpha subunit 60kDa
• Calculated Molecular Weight: 454 aa, 51 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: BC035031
• Gene Symbol: GABPA
• Gene ID (NCBI): 2551
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q06546
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.