GAD1 Polyclonal antibody, PBS Only

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  WB analysis using 10408-1-AP (same clone as 10408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10408-1-AP (GAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10408-1-AP (same clone as 10408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10408-1-AP (GAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  IP result of anti-GAD1 (IP:10408-1-AP, 4ug; Detection:10408-1-AP 1:500) with mouse brain tissue lysate 1920 ug. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 10408-1-AP (same clone as 10408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GAD1 antibody (10408-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GAD1 Polyclonal Antibody for WB, IHC, IF-Fro, IP, ELISA

Cat No. 10408-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-Fro, IP, ELISA

Synonyms

GAD67, GAD, GAD 1, GAD 67, GAD-67

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Product Information

10408-1-PBS targets GAD1 in WB, IHC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0666

  Product name: Recombinant human GAD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-224 aa of BC002815

  Sequence: MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMPSDMRECWLLR

• Full Name: glutamate decarboxylase 1 (brain, 67kDa)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC002815
• Gene Symbol: GAD1
• Gene ID (NCBI): 2571
• RRID: AB_2107733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99259
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.