|Positive WB detected in||mouse brain tissue, rat brain tissue, mouse cerebellum tissue, rat brain brain tissue|
|Positive IP detected in||rat brain tissue|
|Positive IHC detected in||mouse brain tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:100-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21760-1-AP is GAD65 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GAD65 fusion protein Ag16251|
|Full Name||glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)|
|Calculated molecular weight||585 aa, 65 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||BC126327|
|Gene ID (NCBI)||2572|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.
|Product Specific Protocols|
|WB protocol for GAD65 antibody 21760-1-AP||Download protocol|
|IHC protocol for GAD65 antibody 21760-1-AP||Download protocol|
|IF protocol for GAD65 antibody 21760-1-AP||Download protocol|
|IP protocol for GAD65 antibody 21760-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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