GAD65 Polyclonal antibody

GAD65 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21760-1-AP

Synonyms

GAD 65, GAD2, GAD65, Glutamate decarboxylase 2



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, mouse cerebellum tissue, rat brain brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inmouse brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:100-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 21760-1-AP is GAD65 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAD65 fusion protein Ag16251
Full Name glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
Calculated molecular weight 585 aa, 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC126327
Gene symbol GAD2
Gene ID (NCBI) 2572
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.

Protocols

Product Specific Protocols
WB protocol for GAD65 antibody 21760-1-APDownload protocol
IHC protocol for GAD65 antibody 21760-1-APDownload protocol
IF protocol for GAD65 antibody 21760-1-APDownload protocol
IP protocol for GAD65 antibody 21760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease.

Authors - Mehdi Eshraghi
humanWB

Neuropharmacology

Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex.

Authors - Yue Li