GAD65 Polyclonal antibody, PBS Only

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  WB analysis using 21760-1-AP (same clone as 21760-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 21760-1-AP (same clone as 21760-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 21760-1-AP (same clone as 21760-1-PBS)

  IP result of anti-GAD65 (IP:21760-1-AP, 3ug; Detection:21760-1-AP 1:300) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21760-1-AP (GAD65 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21760-1-AP (GAD65 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 21760-1-AP (same clone as 21760-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using GAD65 antibody (21760-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21760-1-AP (same clone as 21760-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAD65 antibody (21760-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21760-1-PBS in a different storage buffer formulation.

GAD65 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 21760-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

GAD2, EC:4.1.1.15, GAD 65, GAD-65

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Product Information

21760-1-PBS targets GAD65 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16251

  Product name: Recombinant human GAD2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-180 aa of BC126327

  Sequence: MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFN

• Full Name: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• Calculated Molecular Weight: 585 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC126327
• Gene Symbol: GAD65
• Gene ID (NCBI): 2572
• RRID: AB_2878916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05329
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.