GAD65 Recombinant antibody, PBS Only

• 

  WB analysis of human cerebellum using 85750-1-RR (same clone as 85750-1-PBS)

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 85750-1-RR (GAD2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85750-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85750-1-RR (same clone as 85750-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85750-1-RR (GAD2 antibody) at dilution of 1:300000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85750-1-PBS in a different storage buffer formulation.

GAD65 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85750-1-PBS

Clone No.250044B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

GAD2, EC:4.1.1.15, GAD 65, GAD-65

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Product Information

85750-1-PBS targets GAD65 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: NM_000818
• Gene Symbol: GAD65
• Gene ID (NCBI): 2572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q05329
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.