GAD65 Recombinant antibody

• 

  WB analysis of human cerebellum using 85750-1-RR

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 85750-1-RR (GAD2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85750-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85750-1-RR (GAD2 antibody) at dilution of 1:300000 incubated at room temperature for 1.5 hours.

GAD65 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85750-1-RR

Clone No.250044B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GAD2, EC:4.1.1.15, GAD 65, GAD-65

---

Tested Applications

• Positive WB detected in: mouse cerebellum tissue, human cerebellum tissue, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85750-1-RR targets GAD65 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: NM_000818
• Gene Symbol: GAD65
• Gene ID (NCBI): 2572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q05329
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.